Iptg x-gal spectinomycin table
WebI: Use the X-gal column to add 0.5 ml of X-gal to each well of the microplate and incubate at 37°C for 2 hours. J: Use the spectinomycin column to add 0.5 ml of spectinomycin to each well of the microplate and incubate at 37°C for 2 hours. A: … WebTo screen bacterial colonies, the chromogenic substrate X-Gal and the gratuitous inducer IPTG are mixed with suitable dilution of a culture, combined with molten top agar, and then spread on agar plates containing the appropriate antibiotic.
Iptg x-gal spectinomycin table
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WebChromoMax IPTG/X-Gal Catalog # 13988100 13988101 13988102 BP42001 BP420010 BP420050 SDS ChromoMax™ IPTG/X-Gal Catalog # 10133443 10225163 10658593 ACRBP4200 BP4200 BP42001 BP420010 BP420050 SDS 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL), Ultrapure, Thermo Scientific Catalog # ALFAAJ10077 SDS … Web4. Post-transformation culture plates After transformation, the bacteria were grown in plates (plates A to D) containing IPTG, X-gal, and the antibiotic spectinomycin. PART 1: 1. What would have happened if we had cultured bacteria from any of the two starter plates in the post-transformation culture plates? 2.
WebFresh E. coli IPTG/X-gal (IX) LB starter plate Fresh E. coli IPTG/X-gal/ARA (IX/ARA) LB starter plate IPTG/X-gal/spectinomycin (IX/SPT) LB plates LB nutrient broth (LB) … WebJan 4, 2024 · The chromogenic substrate X-gal (5-Bromo-4-chloro- 3-indolyl-β- d -galactoside) and IPTG (isopropyl-β- d -thiogalactoside) were used at 40 μg/ml and 50 …
Webchloramphenicol and spectinomycin were added at concentrations of 100 mg/ml, 10 mg/ml, 30 ... respectively. IPTG and X-gal for blue/white selection were added at concentrations of 0.1 mM and 40 µg/ml, respectively. 2.2 Constructing gRNA plasmid and donor DNA To construct gRNA plasmid, a set of primers was used to PCR amplify the pGRB backbone ... WebFig. 2. Blue plaques resulting from different X-Gal and IPTG treatments of electro-transformed E. coli cells. All pictured samples received 10 pg M13mp19 DNA. A) Sample 3, Table 1: electro-transformation plated on an X-Gal and IPTG plate. B) Sample 6, Table 1: 50 µl 20 mg/ml X-Gal and 10 µl 20 mg/ml IPTG added to cells after electroporation.
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WebWhen spreading directly onto agar plates, we recommend 40–50 µl of 40 mg/mL X-gal (2% stock) in dimethylformamide and 30–40 µl of 100 mM IPTG on top of the agar. Let the X … fletchervirus phagesWebIPTG, X-gal, speciinomycin Based on your answers to the previous questions, fill in Table with your predictions of whether there will be bacterial growth on each plate: Okay, So 63 … fletchervirusWebSpectinomycin (40 mL) For a stock solution of 20 mg/mL: 800 mg Spectinomycin 40 mL ddH 2 O Filter sterilize NOTE: Store at 4°C Streptomycin (40 mL) For a stock solution of 50 mg/mL: 2 g Streptomycin 40 mL ddH 2 O Filter sterilize NOTE: Store at 4°C Tetracycline (40 mL) For a stock solution of 2.5 mg/mL: 100 mg Tetracycline 40 mL 50% Ethanol chelsea 1979-80WebLa fusariose vasculaire du cyclamen est une maladie causée par le champignon tellurique Fusarium oxysporum f. sp. cyclaminis. Elle est considérée comme l’une des maladies les plus graves du cyclamen et se traduit par des pertes atteignant jusqu’à 50 % … fletcher village bakery fletcher ncWebApr 4, 2024 · (D) The E. coli K-12 BW25113 WT and ∆ lon strains harboring the plasmid pCA24N-NHis-yefM were induced with 0.5 mM IPTG for 30 min, and 1% spectinomycin (100 µg/mL) was added to the strains to activate a stress response. We collected equivalent quantities of cells at 0 min, 30 min, 60 min and 120 min and then ran Tricine-SDS-PAGE … fletcher vintage glass cutterWebJun 6, 2024 · The growth temperature was 37°C. The antibiotic concentrations used are as follows: 75 µg/mL ampicillin, 50 µg/mL kanamycin, 25 µg/mL chloramphenicol, 50 µg/mL spectinomycin, and 15 µg/mL tetracycline. X-gal and isopropyl β-D-thiogalactopyranoside (IPTG) were used at 25 µg/mL and 0.5 mM, respectively. fletcher village shopping centreWebStudent Guide Table 3 lists the four experimental samples (A, B, C, and D) that you will be working with as well the conditions under which they will be grown. During the activity, … fletcher v mpic